FIXATIVES
SUSA
Acetic acid (Glacial) 4ml
Formalin 20ml
Mercuric chloride 4.5gms
Sodium chloride 0.5gms
Trichloacetic acid 2.0gms
Distilled water 80 ml
Mercury is removed before any staining procedures. The de-waxed slides are immersed in a solution of 0.5% iodine in 70% alcohol for 1 minute followed by immersion in 5% sodium thiosulphate for 1 minute. The slides are then washed in running water for 3 minutes.
10% FORMALIN SOLUTION
10 ml Formalin
90 ml of either Sea water or Distilled water
Other pages of interest.
