STAINING

Bromophenol blue stain for protein

1) Take to 95% alcohol

2) Stain 15 Mins 100 mg Bromophenol blue in 100mls 95% alcohol

3) 20 mins in 0.5% acetic acid

4) Rinse for 3 mins in running water

5) Dehydrate and mount

Bromophenol blue stain on frozen sections

The tissue sections were placed on a glass cover slide and the staining procedure is as follows:

1) 5 min in 100 mg Bromophenol blue in 95% alcohol

2) 1 min in 0.5% acetic acid

3) Rinse in water

4) 95% alcohol 1 min

5) 100% alcohol 1 min

6) Xylene 1 min

7) The glass cover slips were mounted onto glass slides by placing a drop of DPX onto a glass slide which the cover slip was placed. The resultant slide was left to dry (at least overnight)

All Alcohol used in the staining processes was Industrial Mentholated Spirits (I.M.S)